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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withanolides : std mix
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\SM-01-Rep2
Vial No
: 10001
Injection Volume
: 20
Date & Time
: 4/3/07 8:30:25 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.2
0.4
0.6
0.8
17.208
21.458
22.017
23.008
23.883
26.975
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.208
Withanoside IV
569898
7.32
2
21.458
Withanoside V
272130
3.50
3
22.017
Withaferin A
1797785
23.10
4
23.008
Withastramonolide
1787246
22.96
5
23.883
Withanolide A
1842576
23.67
6
26.975
Withanolide B
1513567
19.45
Totals
7783202
100.00