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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 06 lot 10
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S4-Rep1
Vial No
: 10005
Injection Volume
: 20
Date & Time
: 4/4/07 7:12:29 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
17.225
21.500
22.025
23.017
23.908
26.975
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
Withanoside IV
1178151
19.03
2
21.500
Withanoside V
770729
12.45
3
22.025
Withaferin A
1928284
31.15
4
23.017
Withastramonolide
1102671
17.81
5
23.908
Withanolide A
960958
15.52
6
26.975
Withanolide B
249257
4.03
Totals
6190050
100.00