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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 05 lot 20
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S1-Rep3
Vial No
: 10002
Injection Volume
: 20
Date & Time
: 4/4/07 11:01:48 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
17.225
21.408
22.067
23.042
23.908
27.025
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
Withanoside IV
292499
13.99
2
21.408
Withanoside V
182887
8.75
3
22.067
Withaferin A
928052
44.39
4
23.042
Withastramonolide
304876
14.58
5
23.908
Withanolide A
323917
15.49
6
27.025
Withanolide B
58492
2.80
Totals
2090723
100.00