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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 05 lot 21
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S2-Rep1
Vial No
: 10003
Injection Volume
: 20
Date & Time
: 4/4/07 11:47:39 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
0.20
17.225
21.408
22.058
23.033
23.908
27.025
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
Withanoside IV
588934
17.19
2
21.408
Withanoside V
373582
10.90
3
22.058
Withaferin A
1403133
40.95
4
23.033
Withastramonolide
496352
14.49
5
23.908
Withanolide A
465701
13.59
6
27.025
Withanolide B
98415
2.87
Totals
3426117
100.00