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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 06 lot 10
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S4-Rep3
Vial No
: 10005
Injection Volume
: 20
Date & Time
: 4/4/07 8:44:13 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
17.233
21.475
22.050
23.033
23.917
27.017
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.233
Withanoside IV
1165114
18.99
2
21.475
Withanoside V
763045
12.44
3
22.050
Withaferin A
1917315
31.25
4
23.033
Withastramonolide
1075780
17.54
5
23.917
Withanolide A
968374
15.78
6
27.017
Withanolide B
245187
4.00
Totals
6134815
100.00