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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 05 lot 21
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S2-Rep3
Vial No
: 10003
Injection Volume
: 20
Date & Time
: 4/4/07 1:19:18 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
0.20
0.25
17.225
21.417
22.067
23.042
23.908
27.033
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
WIthanoside IV
596170
17.16
2
21.417
Withanoside V
378348
10.89
3
22.067
Withaferin A
1422577
40.95
4
23.042
Withastramonolide
502808
14.47
5
23.908
Withanolide A
473580
13.63
6
27.033
Withanolide B
100847
2.90
Totals
3474330
100.00