148 / 227
QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : RD - 1045
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S5-Rep2
Vial No
: 10006
Injection Volume
: 20
Date & Time
: 4/4/07 2:51:02 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
0.5
17.217
21.400
22.058
23.033
23.908
27.025
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
WIthanoside IV
898123
19.87
2
21.400
Withanoside V
492508
10.90
3
22.058
Withaferin A
1567533
34.68
4
23.033
Withastramonolide
584106
12.92
5
23.908
Withanolide A
807866
17.87
6
27.025
Withanolide B
169826
3.76
Totals
4519962
100.00