146 / 227
QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : RD - 1170
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S6-Rep3
Vial No
: 10007
Injection Volume
: 20
Date & Time
: 4/4/07 5:54:29 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
17.225
21.375
22.092
23.050
23.917
27.058
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
WIthanoside IV
1075861
15.78
2
21.375
Withanoside V
740290
10.86
3
22.092
Withaferin A
2484320
36.43
4
23.050
Withastramonolide
965956
14.17
5
23.917
Withanolide A
1260234
18.48
6
27.058
Withanolide B
292481
4.29
Totals
6819142
100.00