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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : RD - 1045
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S5-Rep3
Vial No
: 10006
Injection Volume
: 20
Date & Time
: 4/4/07 3:36:53 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
0.5
0.6
17.217
21.400
22.067
23.042
23.908
27.042
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
WIthanoside IV
883242
19.68
2
21.400
Withanoside V
487860
10.87
3
22.067
Withaferin A
1557209
34.71
4
23.042
Withastramonolide
585455
13.05
5
23.908
Withanolide A
802542
17.89
6
27.042
Withanolide B
170641
3.80
Totals
4486949
100.00