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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : ERH - 046 : RM
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S8-Rep2
Vial No
: 10009
Injection Volume
: 20
Date & Time
: 4/4/07 3:23:20 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
0.20
0.25
17.225
21.500
22.025
23.017
23.900
26.975
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
Withanoside IV
646445
18.42
2
21.500
Withanoside V
509223
14.51
3
22.025
Withaferin A
1318826
37.57
4
23.017
Withastramonolide
485660
13.84
5
23.900
Withanolide A
438254
12.48
6
26.975
Withanolide B
111940
3.19
Totals
3510348
100.00