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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : ERH - 046 : RM
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S8-Rep3
Vial No
: 10009
Injection Volume
: 20
Date & Time
: 4/4/07 4:09:12 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
0.20
0.25
17.217
21.492
22.017
23.008
23.900
26.967
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
Withanoside IV
654341
18.51
2
21.492
Withanoside V
513695
14.53
3
22.017
Withaferin A
1323105
37.42
4
23.008
Withastramonolide
490071
13.86
5
23.900
Withanolide A
442221
12.51
6
26.967
Withanolide B
112454
3.18
Totals
3535887
100.00