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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : RD - 1162 : RM
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S7-Rep1
Vial No
: 10008
Injection Volume
: 20
Date & Time
: 4/4/07 12:19:51 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.000
0.025
0.050
0.075
0.100
0.125
0.150
17.217
21.508
22.008
23.008
23.900
26.958
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
Withanoside IV
505117
22.86
2
21.508
Withanoside V
298751
13.52
3
22.008
Withaferin A
872090
39.47
4
23.008
Withastramonolide
239104
10.82
5
23.900
Withanolide A
255962
11.59
6
26.958
Withanolide B
38216
1.73
Totals
2209240
100.00