153 / 227
QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : ERH - 046 : RM
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S8-Rep1
Vial No
: 10009
Injection Volume
: 20
Date & Time
: 4/4/07 2:37:28 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
0.20
0.25
17.225
21.508
22.017
23.017
23.908
26.967
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
Withanoside IV
657778
18.46
2
21.508
Withanoside V
517598
14.52
3
22.017
Withaferin A
1338712
37.56
4
23.017
Withastramonolide
492426
13.82
5
23.908
Withanolide A
444196
12.46
6
26.967
Withanolide B
113041
3.17
Totals
3563751
100.00