QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : RD - 1162 : RM
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S7-Rep2
Vial No
: 10008
Injection Volume
: 20
Date & Time
: 4/4/07 1:05:44 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.000
0.025
0.050
0.075
0.100
0.125
0.150
17.217
21.500
22.008
23.008
23.892
26.958
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
Withanoside IV
512636
22.91
2
21.500
Withanoside V
301908
13.49
3
22.008
Withaferin A
880299
39.35
4
23.008
Withastramonolide
243613
10.89
5
23.892
Withanolide A
259210
11.59
6
26.958
Withanolide B
39607
1.77
Totals
2237273
100.00