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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : RD - 1045
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S5-Rep1
Vial No
: 10006
Injection Volume
: 20
Date & Time
: 4/4/07 2:05:10 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
0.5
17.217
21.408
22.058
23.033
23.908
27.025
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
WIthanoside IV
891560
20.04
2
21.408
Withanoside V
482933
10.86
3
22.058
Withaferin A
1539138
34.60
4
23.033
Withastramonolide
572491
12.87
5
23.908
Withanolide A
793283
17.83
6
27.025
Withanolide B
168573
3.79
Totals
4447978
100.00