142 / 227
QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 05 lot 21
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S2-Rep2
Vial No
: 10003
Injection Volume
: 20
Date & Time
: 4/4/07 12:33:26 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
0.20
0.25
17.217
21.408
22.050
23.025
23.900
27.017
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
WIthanoside IV
599396
17.30
2
21.408
Withanoside V
377387
10.89
3
22.050
Withaferin A
1413249
40.80
4
23.025
Withastramonolide
502270
14.50
5
23.900
Withanolide A
471080
13.60
6
27.017
Withanolide B
100538
2.90
Totals
3463920
100.00