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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 05 lot 20
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S1-Rep2
Vial No
: 10002
Injection Volume
: 20
Date & Time
: 4/4/07 10:15:57 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
17.233
21.408
22.083
23.050
23.917
27.033
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.233
Withanoside IV
286199
13.79
2
21.408
Withanoside V
180229
8.69
3
22.083
Withaferin A
927184
44.69
4
23.050
Withastramonolide
300018
14.46
5
23.917
Withanolide A
321506
15.49
6
27.033
Withanolide B
59796
2.88
Totals
2074932
100.00