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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 06 lot 10
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S4-Rep2
Vial No
: 10005
Injection Volume
: 20
Date & Time
: 4/4/07 7:58:21 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
17.225
21.517
22.017
23.017
23.908
26.967
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
Withanoside IV
1150248
18.86
2
21.517
Withanoside V
757348
12.42
3
22.017
Withaferin A
1914204
31.38
4
23.017
Withastramonolide
1090591
17.88
5
23.908
Withanolide A
941431
15.43
6
26.967
Withanolide B
245613
4.03
Totals
6099435
100.00