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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withanolides : std mix
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\SM-01-Rep3
Vial No
: 10001
Injection Volume
: 20
Date & Time
: 4/3/07 9:16:17 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.2
0.4
0.6
0.8
17.217
21.483
22.017
23.008
23.892
26.967
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
Withanoside IV
559628
7.30
2
21.483
Withanoside V
265630
3.46
3
22.017
Withaferin A
1788944
23.34
4
23.008
Withastramonolide
1756420
22.91
5
23.892
Withanolide A
1809961
23.61
6
26.967
Withanolide B
1485590
19.38
Totals
7666173
100.00