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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 06 lot 08
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S3-Rep1
Vial No
: 10004
Injection Volume
: 20
Date & Time
: 4/4/07 4:55:00 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.1
0.2
0.3
0.4
17.217
21.492
22.017
23.008
23.900
26.975
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.217
Withanoside IV
1195002
18.09
2
21.492
Withanoside V
802479
12.15
3
22.017
Withaferin A
2091436
31.67
4
23.008
Withastramonolide
1179907
17.87
5
23.900
Withanolide A
1073657
16.26
6
26.975
Withanolide B
261990
3.97
Totals
6604471
100.00