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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withania somnifera : WS - 05 lot 20
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S1-Rep1
Vial No
: 10002
Injection Volume
: 20
Date & Time
: 4/4/07 9:30:05 AM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.00
0.05
0.10
0.15
0.20
17.225
21.417
22.075
23.050
23.917
27.042
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.225
Withanoside IV
298076
13.93
2
21.417
Withanoside V
187974
8.79
3
22.075
Withaferin A
952512
44.52
4
23.050
Withastramonolide
311700
14.57
5
23.917
Withanolide A
330290
15.44
6
27.042
Withanolide B
58776
2.75
Totals
2139328
100.00