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QUALITY CONTROL DEPARTMENT

Name of Sample

: Withania somnifera : RD - 1170

Method Name

: C:\Methods\WITHANIA.met

Data File Name

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S6-Rep2

Vial No

: 10007

Injection Volume

: 20

Date & Time

: 4/4/07 5:08:37 PM

Minutes

0

5

10

15

20

25

30

35

40

45

Volts

0.0

0.1

0.2

0.3

0.4

17.225

21.392

22.083

23.050

23.917

27.058

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1

17.225

WIthanoside IV

1080249

15.83

2

21.392

Withanoside V

742686

10.88

3

22.083

Withaferin A

2475863

36.29

4

23.050

Withastramonolide

978751

14.34

5

23.917

Withanolide A

1255922

18.41

6

27.058

Withanolide B

289554

4.24

Totals

6823025

100.00