91 / 596
50
P
REPARATION OF THE
3M™M
OLECULAR
D
ETECTION
I
NSTRUMENT
1
4.
Launch the 3M™ Molecular Detection Software and log in.
2
5.
Turn on the 3M Molecular Detection Instrument.
3
6.
Create or edit a run with data for each sample. Refer to the 3M Molecular Detection System
4
User Manual for details.
5
6
NOTE: The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C
7
before inserting the 3M Molecular Detection Speed Loader Tray with reaction tubes. This
8
heating step takes approximately 20 minutes and is indicated by an ORANGE light on the
9
instrument’s status bar. When the instrument is ready to start a run, the status bar will turn
10
GREEN.
11
12
L
YSIS
13
5.
Allow the lysis solution (LS) tubes to warm up by setting the rack at room temperature (20-
14
25 °C) overnight (16-18 hours). Alternatives to equilibrate the LS tubes to room
15
temperature are to set the LS tubes on the laboratory bench for at least 2 hours, incubate
16
the LS tubes in a 37 ±1°C incubator for 1 hour or place them in a dry double block heater
17
for 30 seconds at 100°C.
18
6.
Invert the capped tubes to mix. Proceed to next step within 4 hrs.
19
7.
Remove the enrichment broth from the incubator.
20
8.
One LS tube is required for each sample and the Negative Control (NC) (sterile
21
enrichment medium) sample.
22
8.1
LS tube strips can be cut to desired LS tube number. Select the number of individual LS
23
tubes or 8-tube strips needed. Place the LS tubes in an empty rack.
24
8.2
To avoid cross-contamination, decap one LS tubes strip at a time and use a new pipette
25
tip for each transfer step.
26
4.3 Transfer enriched sample to LS tubes as described below:
27
28
Transfer each enriched sample into an individual LS tube first. Transfer the NC last.
29
30
4.4 Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one LS tube strip -
31
one strip at a time.
32
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only