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51
4.5 Discard the LS tube cap – if lysate will be retained for retest, place the caps into a
1
clean container for re-application after lysis. For processing of retained lysate, see
2
Appendix A.
3
4.6 Transfer 20 µL of sample into a LS tube unless otherwise indicated in Protocol Table
4
2. e.g. Raw dairy products use 10uL.
5
5. Repeat step 4.2 until each individual sample has been added to a corresponding LS tube
6
in the strip.
7
8
9
10
6. Repeat steps 4.1 to 4.6 as needed, for the number of samples to be tested.
11
7. When all samples have been transferred, transfer 20 µL of NC (sterile enrichment medium,
12
e.g., Demi-Fraser Broth) into a LS tube. Do not use water as a NC.
13
8. Verify that the temperature of the 3M Molecular Detection Heat Block Insert is at 100 ±1°C.
14
9. Place the uncovered rack of LS tubes in the 3M Molecular Detection Heat Block Insert and
15
heat for 15 ± 2 minutes. During heating, the LS solution will change from pink (cool) to yellow
16
(hot).
17
Samples that have not been properly heat treated during the assay lysis step may be
18
considered a potential biohazard and should NOT be inserted into the 3M Molecular Detection
19
Instrument.
20
10. Remove the uncovered rack of LS tubes from the heating block and allow to cool in the 3M
21
Molecular Detection Chill Block Insert at least 5 minutes and a maximum of 10 minutes. The
22
3M Molecular Chill Block Insert, used at ambient temperature without the Molecular Detection
23
Chill Block Tray should sit directly on the laboratory bench. When cool, the lysis solution will
24
revert to a pink color.
25
26
11. Remove the rack of LS tubes from the 3M Molecular Detection Chill Block Insert.
27
28
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only