95 / 596
54
Presumptive positive samples should be confirmed as per the laboratory standard operating
1
procedures or by following the appropriate reference method confirmation
(1, 2, 3)
, beginning with
2
transfer from the primary enrichment to secondary enrichment broth (if applicable), followed by
3
subsequent plating and confirmation of isolates using appropriate biochemical and serological
4
methods.
5
NOTE: Even a negative sample will not give a zero reading as the system and 3M Molecular
6
Detection Assay 2 -
Listeria monocytogenes
amplification reagents have a “background” relative
7
light unit (RLU).
8
9
In the rare event of any unusual light output, the algorithm labels this as “Inspect.” 3M
10
recommends the user to repeat the assay for any Inspect samples. If the result continues to be
11
Inspect, proceed to confirmation test using your preferred method or as specified by local
12
regulations
13
14
If you have questions about specific applications or procedures, please visit our website at
15
www.3M.com/foodsafetyor contact your local 3M representative or distributor.
16
17
R
EFERENCES
:
18
7.
US Food and Drug Administration Bacteriological Analysis Manual. Chapter 10: Detection
19
and Enumeration of
Listeria monocytogenes
in Foods. Section C-6. April 2011 Version.
20
8.
US Department of Agriculture (USDA) FSIS Microbiology Laboratory Guidebook 8.08.
21
Isolation and Identification of
Listeria monocytogenes
from Red Meat, Poultry and Egg
22
Products, and Environmental Samples. Effective Date: 6 Nov 2012.
23
9.
ISO 11290-1. Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the
24
Detection and Enumeration of
Listeria monocytogenes
. Amendment 1, 2004-10-15.
25
10.
ISO/IEC 17025. General requirements for the competence of testing and calibration
26
laboratories.
27
11.
ISO 7218. Microbiology of food and animal feeding stuffs – General rules for microbiological
28
examination.
29
12.
ISO 18593. Microbiology of food and animal feeding stuffs – Horizontal methods for
30
sampling techniques from surfaces using contact plates and swabs.
31
32
Appendix A.
Protocol Interruption: Storage and re-testing of heat-treated lysates
33
8.
To store a heat-treated lysate, re-cap the lysis tube with a clean cap (see “L
YSIS
”, 4.5)
34
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only