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Discussion
The 3M
™
MDA 2 -
E. coli
O157 accurately detected
E. coli
O157:H7 from raw ground
beef at 10 hour time point. The MDA 2 -
E. coli
offers the benefits of extremely high
sensitivity and high specificity for the detection of
E. coli
O157 while reducing the overall
time to presumptive results. The MDA 2 -
E. coli
O157 also reduces the total analyst
time required to obtain final results by implementing a single heat lysis step and cool
step, followed by a single transfer to molecular reaction tubes. The small foot print of the
Molecular Detection System requires minimal space, making it easy to move the
instrument or pair additional MDS units to perform multiple MDA runs concurrently. The
updated software is user friendly with easy to interpret results. Also, the ability to
examine Relative Light Unit (RLU) curves makes troubleshooting more streamline if
problems occur during testing. There was no significant, statistical difference observed
at the 10 hour time point for the evaluation of raw ground beef.
Conclusion
The data from the study, within the statistical uncertainty, support the product claims of
the 3M
™
MDA 2 -
E. coli
O157 in the selected food matrix.
The results obtained by the POD analysis of the method comparison study
demonstrated that there were no statistically significant differences between the number
of positive samples detected by the candidate and the reference method for the raw
ground beef food matrix for 10 hour time point.
References
[1] U.S. Department of Agriculture Food Safety and Inspection Service Microbiology
Laboratory Guidebook, Revision 5.09
Isolation and Identification of Escherichia