193 / 453
Page
15
of
17
Raw Ground Beef
3.2 x 10
3
0/5
1
APC conducted in accordance with FDA/BAM Chapter 3
2
E. coli
O157:H7 screen conducted using USDA/FSIS MLG 5.09 lateral flow device.
Table 2: MPN Summary Table for Raw Ground Beef
Raw Ground Beef (73% lean, 27% fat)
E. coli
O157:H7 ATCC 43895
Low Level Inoculum (0.2-2 MPN/Test Portion)
A
B
C
D
E
5 x 650 g
-
-
+
+
+
5 x 130 g
-
+
-
-
+
20 x 325 g (Reference Samples)
7/20
MPN/Test portion
0.49
Low Conf. Limit MPN/Test Portion
0.25
High Conf. Limit MPN/Test Portion
0.85
High Level Inoculum (2-5 MPN/Test Portion)
A
B
C
D
E
5 x 130 g
+
+
+
+
+
5 x 65 g
+
-
+
+
-
5 x 325 g (Reference Samples)
5/5
MPN/Test portion
2.97
Low Conf. Limit MPN/Test Portion
1.30
High Conf. Limit MPN/Test Portion
6.81
*
MPN was calculated for the low level inoculation for raw ground beef using five 650 g, five 130 g and the 20
low level reference method samples (325 g) using the LCF MPN Calculator version 1.6 provided by AOAC-RI
http://www.lcfltd.com/customer/LCFMPNCalculator*
MPN was calculated for the high level inoculation for raw ground beef using five 130 g, five 65 g and the 5
high level reference method samples (325 g) using the LCF MPN Calculator version 1.6 provided by AOAC-RI
http://www.lcfltd.com/customer/LCFMPNCalculatorTable 3: Detailed Results for the 3M™ MDA 2 -
E. coli
O157 in Raw Ground Beef
(73% Lean)
Raw Ground Beef
E. coli
O157:H7 ATCC 43895