298 / 453
QIAGEN
mericon
STEC Workflow Collaborative Study Protocol
May 2016
DRAFT
8
C.
Apparatus
(
a
)
Incubator
.-Capable of maintaining 42 ± 1°C.
(
b
)
Top-loading balance.-
Capable of weighing 1-2000 g.
(
c
)
Laboratory paddle blender.-
Seward 3500 or equivalent.
(
d
)
Vortex mixer.-
Multiple laboratory suppliers (MLS).
(
e
)
Microcentrifuge.-
For 1.5-2.0 mL microcentrifuge tubes.
(
f
)
Pipettors.-
Adjustable, capable of delivering 10-100 μL and 100-1000 μL.
(
g
)
Dry bath incubator.-
Capable of maintaining 100 ± 1°C.
(
h
)
Calibrated thermometer.-
Capable of measuring 100 ± 1°C.
(
i
)
Thermocycler
.-RotorGene Q®, QIAGEN #9001640, with notebook computer containing Rotor-
Gene Q® software version 2.3.1.49 or later.
(
j
)
QIAsymphony® SP.-
QIAGEN #9001297, for automated DNA extraction.
(
k
)
QIAsymphony® AS.-
QIAGEN #9001301, for automated PCR assay setup.
(
l
)
Rotor.-
QIAGEN #9018903, 72 wells, for holding 0.1 mL strip tubes and caps.
(
m
)
Locking ring for 72 well rotor
.-QIAGEN #9018904, for locking strip tubes and caps in 72-well
rotor.
(
n
)
Rotor-Disc 72 Rotor.-
QIAGEN #9018899.
(
o
)
Locking ring for Rotor-Disc 72.-
QIAGEN #9018900.
(
p
)
Reagent cartridge holder (2).-
QIAGEN #997008.
(
q
)
Sample carrier insert, 2 mL v2
.-QIAGEN #9242083, secondary tube adapter for 2 mL screw-cap
tubes.
(
r
)
Cooling adapter.-
QIAsymphony EMT v2, QIAGEN #920730.
(
s
)
Sample preparation cartridges
.-8-well, QIAGEN #997002.
(
t
)
8-Rod covers
.-QIAGEN #997004.
(
u
)
Elution microtubes.-
CL with cap strips, QIAGEN #19588.
(
v
)
Reagent holder cooling adapter.-
QIAGEN #9018090.
(
w
)
Adapter.-
2 x Rotor-Disc, for use with QIAsymphony, QIAGEN #9242204.
(
x
)
Rotor-Disc 72 loading block.-
QIAGEN #9018910.Store refrigerated to ensure that PCR setup is
performed under stable thermal conditions.
(
y
)
Rotor-Disc 72
QIAGEN #981301 (24) or # 981303 (240)
(
z
)
Rotor-Disc heat sealing film
.-QIAGEN #981601 (60) or #981604 (600).
(
aa
)
Rotor-Disc heat sealer
.-QIAGEN #9018898 (110 V) or #9019725 (230 V).
(
bb
)
Filter tips.-
1500 μL, QIAGEN #997024.
(
cc
)
Filter tips.-
200 μL, QIAGEN #990332.
(
dd
)
Filter tips.-
50 μL, QIAGEN #997120.
D.
Additional Supplies, Reagents and Media
(
a
)
Filter laboratory blender bags.-
MLS.
(
b
)
Serological pipettes –
Aerosol resistant
(
c
)
Micropipette tips,
aerosol resistant.-MLS.
OMAMAN-36 B : Collaborative Study Protocol
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only