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It is recommended that the 3M Molecular Detection Assay
Listeria
method be adopted as Official
2
First Action status for the detection of
Listeria
species in selected foods including beef hot dogs
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(25 g), deli turkey (25 g), cold smoked salmon (25 g), full fat cottage cheese (25 g), and two
4
environmental surfaces sealed concrete (sponge in 100 mL and sponge in 225 mL enrichment
5
volume) and stainless steel (swab in 10 mL and sponge in 225 mL enrichment volume) and
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plastic (swab in 10 mL) enriched in Demi-Fraser broth base.
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Acknowledgements
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10
We would like to extend a sincere thank you to the following collaborators for their dedicated
11
participation in this study:
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Peyman Fatemi and Sharon Spencer– Aemtek, Inc., Fremont, CA.
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Joel Blumfield – EDL Labs, Inc., Purvis, MS.
15
Adam Hankins-McCoy and McCoy Laboratories, Inc., Madisonville, Ky.,
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Rob Dermer, Natalie Shipley and James Williams– Microbac Laboratories, Inc., Fayetteville,
17
NC.
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Ashley Morris, Robert Brooks and Karen Powers – Microbac Laboratories, Inc., Maryville, TN.
19
Jerry Lynn Picket and Amanda Thielen - Tyson WBA Analytical, Springdale, AR
20
Leslie Thompson – VANGUARD SCIENCES, North Sioux City, SD
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Chris Lopez, Alex Brandt and Bharath Brahmanda – Food Safety Net Services, San Antonio, TX
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Luci Hardrath – AgSource Laboratories, Marshfield, WI
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Yi Chen and Anna Laasri- U.S Food and Drug Administration, Center for Food Safety and
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Applied Nutrition, College Park, MD
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Robert Brooks – ATC Microbiology, LLC., North Little Rock, AR
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Diane Wood and Andrew Sweet – Maple Leaf Consumer Foods Guelph, Ontario, Canada
27
Jean Schoeni - Covance, Inc., Madison, WI
28
Brian Kupskiand Nicole Cuthbert – Silliker, Food Science Center, Crete, IL
29
Ben Bastin– Q Laboratories Inc., Cincinnati, OH
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We would like to extend a special thanks to the following team members at Q Laboratories,
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Inc. for their efforts during the collaborative study: M. Joseph Benzinger Jr., Allison Mastalerz,
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Kiel Fisher, Kateland Koch, Will Judd and Nicole Klass
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References
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(1) Chen, Yi. (2012) FDA - Bad Bug Book – Foodborne Pathogenic Microorganisms and
40
Natural Toxins,
Listeria monocytogenes
species. 2
nd
Ed.
41
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(2) Center for Disease Control and Prevention–
http://www.cdc.gov/listeria/ (Accessed
43
September 2014)
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(3) New Zealand Ministry of Health;
Listeria monocytogenes Fact Sheet.
May 2001.
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http://www.foodsafety.govt.nz/elibrary/industry/Listeria_Monocytogenes-Science_Research.pdf .47
(Accessed December 2014)
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Candidates for 2016 Method of the Year
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