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Based on the data presented, the repeatability values obtained at all incubation conditions and
1
for all three contamination levels was generally similar between the two methods.
2
For raw frozen ground beef patties and raw almonds, the 3M Petrifilm RYM Count Plate
3
was more repeatable than the FDA BAM/ISO 21527 reference methods for 1 of the 6
4
contamination levels analyzed at 25
o
C and 4 of the 6 contamination levels analyzed at 28
o
C.
5
6
Recommendations
7
8
It is recommended that the 3M Petrifilm Rapid Yeast and Mold Count (RYM) Plate be
9
adopted as Official First Action status for the enumeration of yeast and molds in the following
10
high water activity matrices: yogurt (1.5% fat), frozen bread dough, fermented salami, sour
11
cream, ready-made pie, raw frozen ground beef patties (77%), ready-to-eat deli sandwiches,
12
sliced apples and the following low water activity matrices: raw almonds and dehydrated soup.
13
14
15
Acknowledgements
16
17
We would like to extend a sincere thank you to the following collaborators for their dedicated
18
participation in this study:
19
20
Dave Metzger, Wendy Lee and Thomas Hirsch – Accelerated Analytical, Milwaukee, WI.
21
Peyman Fatemi and Sharon Spencer– Aemtek, Inc., Fremont, CA.
22
Joel Blumfield – EDL Labs, Inc., Purvis, MS.
23
Bryan Dieckleman – Eurofins Microbiology Laboratories, Inc., Des Moines, IA.
24
Karilyn Gonzales and Chelsea Fagundes– Food Microbiological Laboratories, Inc., Cypress, CA.
25
Jerry Reed & Jeraldine Medellin –Metrix 360 Laboratories, San Antonio, TX.
26
Adam Hankins, McCoy and McCoy Laboratories, Inc., Madisonville, Ky.,
27
Rob Dermer, Natalie Shipley and James Williams– Microbac Laboratories, Inc., Fayetteville,
28
NC.
29
Ashley Morris, Robert Brooks and Karen Powers – Microbac Laboratories, Inc., Maryville, TN.
30
Brenda Brown – Murray Brown Laboratories, LLC, Denver, CO.
31
Karen Beers- Safe Foods Corporation, Rogers, AR
32
Alexandra Calle – Texas Tech University, Food Safety and Microbiology, Lubbock, TX.
33
Jerry Lynn Picket and Joseph Reynolds - Tyson WBA Analytical, Springdale, AR
34
Andy Gaydos – Waters Agricultural, Camilla, GA.
35
Allison Mastalerz– Q Laboratories Inc., Cincinnati, OH
36
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We would like to extend a special thanks to the following team members at Q Laboratories,
38
Inc. for their efforts during the collaborative study: M. Joseph Benzinger Jr., Ben Bastin, Kiel
39
Fisher, Kateland Koch, Will Judd and Nicole Klass
40
41
42
References
43
44
(1) Tournas, Valerie, et al. U.S Food and Drug Administration – Bacteriological Analytical
45
Manual, Chapter 18
Yeasts, Molds and Mycotoxins.
2001,
46
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm071435.htm47
(Accessed, July 2014)
48
49
Candidates for 2016 Method of the Year
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