102-103 / 471
Table 2. Total Yeast and Mold Log
10
counts for frozen raw ground beef patties by 3M Petrifilm RYM Count Plate
after 48 hours incubation at 25
o
C vs. FDA BAM and ISO 21527 reference methods
a
Uninoculated Control
Low
Medium
High
Petrifilm
RYM
FDA BAM/
ISO 21527
e
Petrifilm
RYM
FDA BAM/
ISO 21527
e
Petrifilm RYM
FDA BAM/
ISO 21527
e
Petrifilm
RYM
FDA BAM/
ISO 21527
e
Lab
A
b
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
1
<1.00
<1.00
<1.00
<1.00
2.18
1.78
2.23
1.60
3.45
3.38
3.49
3.32
4.64
4.62
4.32
4.54
2
<1.00
<1.00
<1.00
<1.00
2.43
2.48
2.43
2.83
3.62
3.71
3.49
3.45
4.62
4.73
4.53
4.52
3
<1.00
<1.00
<1.00
<1.00
2.34
2.30
2.41
2.32
3.52
3.30
3.63
3.53
4.64
4.40
4.82
4.81
4
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5
<1.00
<1.00
<1.00
<1.00
1.95
1.78
1.70
2.00
3.41
3.53
3.30
3.41
4.43
4.51
4.46
4.64
6
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
7
<1.00
<1.00
<1.00
<1.00
2.23
2.36
1.80
1.82
3.51
3.56
3.36
3.61
4.79
4.32
4.43
4.49
8
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
9
<1.00
<1.00
<1.00
<1.00
2.45
2.23
2.28
2.23
3.56
3.60
3.51
3.45
4.72
4.73
4.57
4.79
10
<1.00
<1.00
<1.00
<1.00
2.20
2.20
2.11
2.00
3.60
3.45
3.45
3.43
4.76
4.61
4.66
4.40
11
<1.00
<1.00
<1.00
<1.00
2.36
1.00
2.40
1.90
3.57
3.66
3.49
3.54
4.68
4.56
4.49
4.59
12
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
13
<1.00
<1.00
<1.00
<1.00
2.45
2.08
2.00
1.67
3.49
3.51
3.52
3.48
4.75
4.89
4.67
4.77
14
<1.00
<1.00
<1.00
<1.00
2.20
1.00
2.34
c
1.00
c
3.41
3.67
3.57
3.70
4.70
4.60
4.65
4.62
15
<1.00
<1.00
<1.00
<1.00
2.30
2.28
2.15
2.23
3.38
3.53
3.38
3.23
4.72
4.85
4.61
4.66
RYM= 3M Petrifilm Rapid Yeast and Mold method.
FDA BAM= FDA BAM Chapter 18 method for enumeration of yeasts, molds and mycotoxins.
ISO 21527= ISO 21527:2008 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration for yeast and molds – Part 1: Colony
count technique in products with water activity greater than 0,95 [4] and Part 2: Colony count technique in products with water activity less than or equal to
0,95
a
Log
10
yeast and mold CFU/g;
b
A and B= Indicated duplicate test portions;
c
Cochran’s outlier
d
Laboratory data not included in statistical analysis for all contamination levels
e
FDA BAM and ISO 21257 reference method replicates were enumerated at 5 and 7 days (uninoculated test portions) of incubation at 25
o
C
1
2
Candidates for 2016 Method of the Year
88