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Primary and Secondary Evaluation of Method OMAMAN-06
OMAMAN-06:
DETERMINATION OF PANTOTHENIC ACID IN FORTIFIED BOVINE MILK-BASED
INFANT FORMULA POWDER, FORTIFIED SOYA-BASED INFANT FORMULA
POWDER, FORTIFIED CEREALS, UNFORTIFIED CEREALS, VITAMIN TABLETS AND
DIETARY SUPPLEMENTS BY SURFACE PLASMON RESONANCE: COLLABORATIVE
STUDY
Author(s):
Dr. Anthony O'Kane, MSB, Institute for Global Food Security (IGFS), Queens
University Belfast
Primary Reviewer:
Shang-Jing (Jean) Pan
Secondary Reviewer
: John Austad
S
UMMARY OF
M
ETHOD
:
This is an inhibition assay, pantothenic acid binds to
poly (A)-binding protein (
PABP) and inhibits the
interaction between PABP and immobilized PA on sensor chip. PABP binds to PA on sensor chip and
generates surface plasmon resonance (SPR) response which is inversely related to PA level in sample.
Using a simple sample preparation, Vitamin B5 (Pantothenic Acid) can be determined using SPR that
uses the specificity of the ligand binding protein interaction to determine the concentration Pantothenic
Acid.
G
ENERAL
C
OMMENTS
:
This method is a quick assay, more time-efficient than micro-assay and chromatography based methods.
Due to the use of proprietary instrumentation and kits, it limits the number of vendors a laboratory
could use to purchase. The manuscript indicates the costs are less than HPLC and MS instrumentation,
but no actual costs are used. There is limited applicability of the instrument.
P
ROS
/S
TRENGTHS
:
This method is very fast and there is a quick determination of B5 in specified matrices versus legacy
microbiological methods.
C
ONS
/W
EAKNESSES
:
It is a proprietary assay and it has a limited scope. The method only uses specific instrumentation and
kits. It was also noted that there are high RSDs in some of the matrices.
EXPERT REVIEW PANEL VOTE
AND
RECOMMENDATION
MOTION: Not to move forward for First Action Official Methods
status.
Entered By:
Austad
Second By:
Pan
Vote:
7
Approved
0
Opposed
0
Abstain
M
OTION PASSED
ERP PROFILE SUMMARIES
194