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Background
Summary of current methods used in Aloe
qualification (identification) and quantitation analysis
(
cont’d
):
•
Example of aloe identification by
1
H NMR
Isocitric acid lactone
Isocitric acid lactone
Isocitric acid
Isocitric acid
Aloe inner gel
Aloe whole leaf
Acetyl groups
Unique fingerprint
only found in aloe
acetylated
polysaccharides
Background
Summary of current methods used in Aloe
qualification (identification) and quantitation analysis
(
cont’d
):
•
HPLC – qualification of aloe organic acid fingerprint, including
malic, lactic, citric, fumaric acid, isocitric, and isocitric acid
lactone. Isocitric and its lactone are whole leaf markers;
•
Example of aloe HPLC fingerprint for identification;
Whole leaf
Inner gel
Rind