SPDS eBook March 2017

Background

Summary of current methods used in Aloe

quantitation analysis:

•

H NMR – polysaccharides, monosaccharides, organic

acids;

•

HPLC – organic acids;

•

HPAEC‐PAC – organic acids, disaccharides,

monosaccharide, oligosaccharides;

•

GC – organic acids, monosaccharides including existed

monosaccharides or hydrolyzed from polysaccharides;

•

Colorimetric – quantitation of aloe polysaccharides by

photometric analysis;

Background

Summary of current methods used in Aloe

quantitation analysis (

cont’d

•

GPC‐RI (Reflective Index)

–

Provide fingerprint of aloe polysaccharides and their

molecular weight and size;

–

Require polysaccharide standards, such as dextran,

pullulan;

•

GPC‐RI‐MALLS (Multi Angle Light Scattering)

–

Measure absolute molecular weight;

–

Don’t require polysaccharide standards for quantitation;