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Background
Summary of current methods used in Aloe quantitation
analysis (
cont’d
):
•
1
H NMR vs. GPC‐RI‐MALLS
–
NMR quantitation only works on the acetylated polysaccharides;
–
Degrees of acetylation on the aloe polysaccharides are varied
depending on manufacturing process;
–
GPC‐RI‐MALLS quantitation covers all the polymers eluted from GPC
including acetylated or non‐acetylated polysaccharides or other
polymers such as proteins;
SMPR of Aloe Identification Key Points
•
Identification of acetylated glucomannan
polysaccharides derived from Aloe Vera in dietary
ingredients and dietary supplements;
•
Candidate methods should be able to differentiate
acetylated glucomannan polysaccharides derived
from whole leaf and/or inner leaf products from gel;
•
Any analytical technique that meets the method
performance requirements is acceptable;
•
May require developing aloe polysaccharide
standards for qualification;