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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
13
C
G
= C
GB
× D × (V/m) × 0.0001
C
F
= C
FB
× D × (V/m) × 0.0001
TF = (C
F
× 0.9) + C
G
Where,
TF = Total fructan in sample (in g/100 g)
C
G
= Glucose released from fructan (in g/100 g)
C
GB
= Glucose concentration in Solution B (in µg/mL)
C
F
= Fructose released from fructan (in g/100 g)
C
FB
= Fructose concentration in Solution B (in µg/mL)
D = Dilution factor between Solution A and solution B (from Table)
V = Total volume of Solution A (in mL)
m = Amount of sample weighed to prepare Solution A (in g)
0.001 = factor to convert analyte concentration in solution (in µg/mL) to analyte concentration in
sample (in g/100 g)
0.9 = factor to correct for uptake of water during fructan hydrolysis
For samples with low fructan content requiring the blank correction adapt the above equations as
follows:‐
C
G
= (C
GB
– C
G0
) × D × (V/m) × 0.0001
C
F
= (C
FB
– C
F0
) × D × (V/m) × 0.0001
Where
C
G0
= Glucose concentration in Blank Solution B (in µg/mL)
C
F0
= Fructose concentration in Blank Solution B (in µg/mL)
2.3. Validation Design
2.3.1
Linearity
Calibration solutions of glucose (2 µg/mL – 300 µg/mL) and fructose (20 µg/mL – 1100 µg/mL) at 8
different levels, were prepared in triplicate all containing the chitobiose internal standard (at 600
µg/mL). The ratio of the peak areas (analyte / chitobiose) was plotted against the concentration of
analyte (since the chitobiose concentration remained constant throughout there was no need to
plot the ratio of the concentrations on the x‐axis). A quadratic model was used to fit the data for
calibration purposes.
The relative residuals were calculated and plotted against the analyte concentration.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE