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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
15
Table 4: Design of Spike-Recovery Experiment Using Three Fructan Ingredients in Six SPIFAN Matrices
Nestlé
Code
Sample
Level 0
(0 g/100g)
Level 1
(0.03 g/100g)
Level 2
(2 g/100g)
Level 3
(5.0 g/100g)
KBI‐00048 Infant Formula Powder Milk
Based
none
Orafti P95
Orafti HP
NutraFlora P95
KBI‐00052
Infant Formula Powder Soy
Based
none
Orafti HP
NutraFlora P95
Orafti P95
KBI‐00050
Adult Nutritional RTF High
Protein
none
NutraFlora P95
Orafti P95
Orafti HP
KBI‐00045
Adult Nutritional Powder
Low Fat
none
Orafti P95
Orafti HP
NutraFlora P95
KBI‐00033
Infant Formula Powder
Partially Hydrolyzed Milk
Based
none
Orafti HP
NutraFlora P95
Orafti P95
KBI‐00047
Infant Elemantal Powder
none
NutraFlora P95
Orafti P95
Orafti HP
2.3.4
Limits of Detection and Quantification
Limits of detection and quantification were determined by analyzing a blank sample (KBI‐00052,
Infant formula Powder Soy Based) 14 times (7 days in duplicate). The sample has minor signals at
the expected retention times of glucose and fructose. They have been determined as fructan and
the data used to calculate LoD and LoQ by taking the mean fructan content determined by the 14
analyses and adding 3 times the standard deviation to estimate LoD, and adding 10 times the
standard deviation to estimate LoQ.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE