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Use the standard curve to calculate the molar concentration (in µmol/mL) of each oligosaccharide
in the chromatogram (C
m
) with enzyme treatment, and calculate the total oligosaccharides in that
sample as follows:-
Then calculate the GOS content of sample as follows:-
Where,
C
TOS
= Total concentration of oligosaccharides in the untreated sample (in g/100 g)
C
B
= Total concentration of oligosaccharides in the enzyme-treated sample (in g/100 g)
C
GOS
= Total concentration of GOS in the sample (in g/100 g)
C
m
= molar concentration of each individual oligosaccharide in the sample (in µmol/mL)
MW = molecular weight of each individual oligosaccharide in the sample
V = volume to which the original sample weight was diluted (in mL)
m = weight of sample diluted to volume (V) (in g)
0.0001 = factor to convert result from µg/g to g/100 g.
METHOD
FOR ERP USE ONLY
DO NOT DISTRIBUTE