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Single Lab Validation of a Method for the Determination of β‐
Galactooligosaccharides in Infant Formula & Adult Nutritionals / Sean Austin (NRC,
Lausanne)
25 Jul 2016
CONFIDENTIAL
∙ This document may not be reproduced or disclosed to third parties without prior authorization
Page 4 / 35
2 M E T H O D O L O G Y A N D T R I A L S
2.1.
Samples
A SPIFAN single laboratory validation material test kit was used. The kit contains representative
products covering the scope of the project as well as a standard reference material (SRM 1849a).
The kit contains 5 products described as placebo products and 14 products described as fortified
products. Four of the fortified products were found to contain GOS. It was also found that one of
the placebo products contained GOS. In addition to the SPIFAN matrices, one Nestlé reference
sample was analysed, and GOS‐containing products from two other companies were purchased in
the supermarket to extend the total number of samples. The Nestlé reference sample was analysed
without prior reconstitution.
Table 1: Samples Used In This Study
Product Description
Lot No
NRC Reference
Placebo Products
1. Child Formula Powder
00847RF00
KBI‐00020
2. Infant Elemental Powder
00796RF
KBI‐00028
3. Adult Nutritional RTF, High Protein
00821RF00
KBI‐00029
4. Adult Nutritional RTF, High Fat
00820RF00
KBI‐00030
5. Infant Formula RTF, Milk Based
EV4H2Q
KBI‐00031
Fortified Products
1. SRM 1849a
CLC10‐b
KBI‐00032
2. Infant Formula Powder Partially Hydrolyzed Milk
Based
410057652Z
KBI‐00033
3. Infant Formula Powder Partially Hydrolyzed Soy
Based
410457651Z
KBI‐00034
4. Toddler Formula Powder Milk Based
4052755861
KBI‐00035
5. Infant Formula Powder Milk Based
4044755861
KBI‐00044
6. Adult Nutritional Powder Low Fat
00859RF00
KBI‐00045
7. Child Formula Powder
00866RF00
KBI‐00046
8. Infant Elemental Powder
00795RF
KBI‐00047
9. Infant Formula Powder FOS/GOS Based
50350017W1
KBI‐00053
10. Infant Formula Powder Milk Based
K16NTAV
KBI‐00048
11. Infant Formula Powder Soy Based
E10NWZC
KBI‐00052
12. Infant Formula RTF Milk Based
EV4H2R
KBI‐00049
13. Adult Nutritional RTF High Protein
00730RF00
KBI‐00050 / L3B‐00037
14. Adult Nutritional RTF High Fat
00729RF00
KBI‐00051
Nestlé Products
1. Infant formula powder with GOS/FOS
Ref 56A
Ref 56A
Additional Products
1. Infant formula with GOS
L15515
L4B‐00006
2. Infant formula with GOS/FOS
F 0135742031 2040 006394
L4B‐00007
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE