36 / 220
LC-MS/MS Analysis of Nicotinic acid
and Nicotinamide
Chemistry Method
Version 2
Page 11/15
Prepared by
S. Bhandari
Approved by
Issue Date
Implementation Date
________________________________________________________________________________
Draft Date: 3/2/16 Copyright © Merieux NutriSciences, 2016
Uncontrolled When Printed
17.
18.
(
d
)
Flow rate. —
0.30 - 0.60 mL/min.
19.
(
e
)
Injection volume. —
2.0
L.
20.
(
f
)
Temperature. —
20
o
C.
21.
(
g
)
Autosampler temperature. —
5.0
o
C.
22.
(
h
)
Run time. —
21 min.
23.
ABSciex 5500 QTrap / ABSciex 6500 QTrap
24.
25. CUR – 30
26. CAD – medium
27.
IonSpray Voltage IS – 4250
28. Temperature – 550
29. GS1 – 70
30. GS2 – 50
ID
Q1 Mass
(Da)
Q3 Mass
(Da)
Time
(msec)
DP (volts)
EP (volts)
CE (volts)
CXP (
volts)
B3-acid_Quant
124.3
78.1
20
50
10
28
9
B3-acid_Qual1
124.3
80
20
50
10
28
10
B3-acid_Qual2
124.3
53
20
50
10
29
10
B3-amide_Quant
123.3
80.1
20
45
10
27
10
METHOD
FOR ERP USE ONLY
DO NOT DISTRIBUTE