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LC-MS/MS Analysis of Nicotinic acid
and Nicotinamide
Chemistry Method
Version 2
Page 13/15
Prepared by
S. Bhandari
Approved by
Issue Date
Implementation Date
________________________________________________________________________________
Draft Date: 3/2/16 Copyright © Merieux NutriSciences, 2016
Uncontrolled When Printed
Adjusted analyte conc. (ng/mL) in sample or std. as asample = analyte in
conc. sample or std. as a sample (ng/mL)
–
Respective analyte level in the
blank (ng/mL).
4.9.3. Calculation of each of the analyte in the samples:
1. Concentration of each of the analyte is calculated in each sample is calculated by
the following relationship:
Conc. of the analyte in the sample (mg/Kg) = Adjusted analyte concentration
(ng/mL) in the sample extract x Dilution of the extract (fold) x Final Volume (mL)
of the extract/ (Sample Aliquot (g) x 1000).
B3 (niacin and niacinamide) calculated in the sample are for the free base form.
4.9.6 Calculation of total vitamin B3 (Niacin) in samples:
The niacinamide estimated in the sample is calculated as niacin by multiplying
niacinamide (mg/kg) estimated value by the factor provided in the Table
Niacinamide in the sample is calculated as niacin by the following relationship.
Niacinamde as Niacin (mg/kg) = Niacinamide mg/kg x 1.008
Vitamin B3 Conversion Factors
Vitamin
Abbreviation
Salt to make
standard
Mol. Wt of used
std.
to report
Nicotinamide as
Nicotinic acid -
Multiply by
to report total niacin
as nicotinic acid
Nicotinic Acid
Vitamin B3-Acid
Free Base
123.11
1.0
sum Niacin +
Niacinamide*1.008
Nicotinamide
Vitamin B3-NH
Free Base
122.13
1.0080
Total Niacin (mg/kg) in the sample is calculated by sum of of niacin mg/kg and niacinamide calculate as niacin
(mg/kg. This is also expressed ion the following relationship.
Total Noacin in the sample (mg/kg) = Niacin (mg/kg) + Niacinamide calculated as niacin (mg/kg).
METHOD
FOR ERP USE ONLY
DO NOT DISTRIBUTE