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New Member Welcome Coffee
10:15 am - 11:15 am, Room 404AB
All new and prospective Biophysical Society members are invited to par-
ticipate in an informal gathering to meet members of the Society’s coun-
cil and committees, find out about the Society’s activities, get acquainted
with other new members, and enjoy refreshments. Current members are
encouraged to come meet the new members.
Exhibitor Presentation
Wyatt Technology Corporation
10:30 am - 12:00 pm, Room 513
Get it Right the First Time - Enhancing Protein Binding and Structural
Studies with the Light-Scattering Toolkit
Biophysical binding studies utilizing surface plasmon resonance (SPR),
biolayer interferometry (BLI), isothermal titration calorimetry (ITC), and
related techniques are central to the study of protein-protein, protein-
DNA and similar biomolecular interactions. Though these are well-estab-
lished techniques, in a variety of circumstances, binding measurements
may be ambiguous or even fail to provide useful data. Wasted measure-
ments can end up being costly in terms of consumables and time.
Small-angle X-ray scattering (SAXS) and small-angle neutron scattering
(SANS) are powerful techniques for studying biomolecular structure.
SAXS and SANS usually require precious beam time at large facilities, leav-
ing little room for error where the sample preparation is concerned. Poor
samples provide poor SAXS/SANS data, but the opportunity to utilize the
X-ray or neutron beammay never be recovered.
One thing that SAXS and SANS have in common with SPR, BLI and ITC, is
the urgent need to verify sample quality and aggregation state in solution
prior to carrying out structural or binding measurements. This seminar
discusses a suite of complementary techniques, all based on light scat-
tering, that are useful in assessing and troubleshooting many of the un-
derlying characterization issues. Multi-angle light scattering (MALS) and
dynamic light scattering (DLS) can help researchers assess solution quality
prior to running binding or structural experiments, qualify aggregation
behavior of analytes, and characterize complex interactions that may not
be amenable to standard characterization methodology. Judicious use of
the biophysical light-scattering toolkit is essential for robust and reliable
interaction and structure studies.
Speaker
Sophia Kenrick, Application Scientist, Wyatt Technology Corporation
Symposium
Mechanosending and Mechanosignaling in
Muscle
10:45 am - 12:45 pm, Petree Hall C
Chair
Olga Mayans, University of Konstanz, Germany
923-Symp10:45 am
MECHANOSENSITIVE STRUCTURAL STATES OF TITIN. Zsolt Mártonfalvi,
Pasquale Bianco, Katalin Naftz, Dorina Kőszegi, György Ferenczy,
Miklós S.
Kellermayer
924-Symp11:15 am
TITIN(S): TOWARDS AN ATOMIC UNDERSTANDING OF MECHANOSEN-
SORY EVENTS IN THE ELASTIC SCAFFOLDS OF THE MUSCLE SARCOMERE.
Olga Mayans
, Jennifer Fleming, Rhys Williams, Barbara Franke, Hang Lu,
Guy Berrian
925-Symp11:45 am
MECHANO-CHEMO-TRANSDUCTION IN CARDIOMYOCYTES DURING BEAT-
TO BEAT CONTRACTION UNDER MECHANICAL LOAD.
Ye Chen-Izu
926-Symp12:15 pm
DETYROSINATED MICROTUBULES BEAR LOAD AND TRANSMIT MECHANI-
CAL FORCE IN CARDIOMYOCYTES. Patrick Robison, Matthew Caporizzo,
Alexey Bogush, Kenneth Margulies,
Benjamin Prosser
Symposium
Future of Biophysics
10:45 am - 12:45 pm, Petree Hall D
Co-Chairs
Vasanthi Jayaraman, University of Texas Healtah Science Center
E. Michael Ostap, University of Pennsylvania
No Abstract
10:45 am
ENGINEERING BIOMIMETIC MATRICES TO UNDERSTAND BRAIN/TUMOR
INTERACTIONS DURING METASTASIS.
Kimberly Stroka
No Abstract
11:15 am
COMBINATORIAL REGULATION OF BAR DOMAIN PROTEINS AT THE INTER-
FACE BETWEEN THE CYTOSKELETON AND MEMBRANES.
David Kast
No Abstract
11:45 am
NASCENT AND ANTIMICROBIAL PEPTIDES THAT TARGET THE RIBOSOMAL
EXIT TUNNEL TO BLOCK PROTEIN SYNTHESIS IN BACTERIA.
Axel Innis
No Abstract
12:15 pm
MECHANISM OF GATING AND ION SELECTIVITY IN ASIC/ENAC/DEG
CHANNELS.
Isabelle Baconguis
Platform
Voltage-gated K Channels and Mechanisms of
Voltage Sensing and Gating I
10:45 am - 12:45 pm, Room 502A
Co-Chairs
Peter Larsson, University of Miami
Gail Robertson, University of Wisconsin-Madison
927-Plat
10:45 am
N-ARACHIDONOYL TAURINE RESCUES DIVERSE LONG QT SYNDROME-
ASSOCIATED MUTATIONS IN THE CARDIAC IKS CHANNEL.
Sara I. Liin
,
Johan E. Larsson, Rene Barro-Soria, Mark A. Skarsfeldt, Bo H. Bentzen, H
Peter Larsson
928-Plat
11:00 am
THE F-ACTIN BINDING PROTEIN TRIOBP-1 REGULATES HERG K
+
CHAN-
NELS.
Ashley A. Johnson
, Dave Jones, Elon C. Roti Roti, Gail Robertson,
Matthew Trudeau
929-Plat
11:15 am
A NOVEL SITE OF COMPETITIVE PIP2 AND CALMODULIN INTERACTION
TO KCNQ1 C-TERMINUS HELIX B IS CRUCIAL FOR IKS CHANNEL ACTIVITY.
William S. Tobelaim
, Meidan Dvir, Guy Lebel, Meng Cui, Tal Buki, Asher
Peretz, Diomedes Logothetis, Joel Hirsch, Bernard Attali
930-Plat
11:30 am
MOLECULAR DYNAMICS SIMULATIONS OF KIR2.2-CHOLESTEROL INTER-
ACTIONS.
Nicolas Barbera
, Manuela A. Ayee, Belinda S. Akpa, Irena
Levitan
931-Plat
11:45 am
CRYO-EM STRUCTURE OF THE BK ION CHANNEL IN A LIPID MEMBRANE.
Liguo Wang
, Lige Tonggu, Xi Zhan