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© 2015 AOAC INTERNATIONAL
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additions.
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Acetate buffer (100 mM, pH 5.0)
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Bacillus
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Based on Bacterial Amylase Unit (BAU) method.
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dry matter basis. To test for interference from release of glucose
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of these substrates should be less than 0.5% on a dry matter basis
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must not contain appreciable concentrations of glucose (<0.5%),
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measurements.
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Diluted amyloglucosidase solution.
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amyloglucosidase with 100 mM sodium acetate buffer,
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Pipet concentrated amyloglucosidase into buffer, rinsing tip by
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amyloglucosidase used should not contain greater than 0.5%
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Aspergillus niger
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Benzoic acid solution (0.2%)
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GOPOD reagent.
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Mixture of glucose oxidase,
7000 U/L, free from catalase activity; peroxidase, 7000 U/L;
and 4-aminoantipyrine, 0.74 mM
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life: 1 month. Before use in test sample determinations, determine a
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Glucose working standard solutions.
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solutions. Prepare solutions at least one day before use to allow