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M
ASTOVSKA
ET AL
.:
J
OURNAL OF
AOAC I
NTERNATIONAL
V
OL
.
98, N
O
. 2, 2015
483
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and fat, which are dependent on the silica deactivation. The
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See
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2014.08A
for
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B. Apparatus
(
a
)
Homogenizer
²:$5,1* EOHQGHU 0RGHO
%/
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(
b
)
Solvent evaporator
.—Any suitable solvent evaporator,
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HYDSRUDWRU RU D QLWURJHQ EORZ GRZQ V\VWHP PD\ EH XVHG DV
ORQJ DV LW SURYLGHV UHVXOWV PHHWLQJ WKH ODERUDWRU\ TXDOL¿FDWLRQ
PHWKRG VHW XS UHTXLUHPHQWV DEVROXWH DQDO\WH UHFRYHULHV !
in both evaporation steps).
(
c
)
Centrifuge
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! UFI IRU PLQ
(
d
)
Furnace/oven
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& RSHUDWLRQ
.
(
e
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Balance(s)
.—Analytical,
capable
of
accurately
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(
f
)
Gas chromatograph-mass spectrometer
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requirements (to provide reliable results for the calibration
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2014.08A
).
(
g
)
GC column
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other column that enables adequate separation of PAHs as
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see
G
).
C. Reagents and Materials
(
a
)
Hexane.—
!
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(
b
)
Isooctane.—
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(
c
)
Ethyl acetate.—
!
IRU *& UHVLGXH DQDO\VLV
(
d
)
Dichloromethane.—
IRU *& UHVLGXH DQDO\VLV
(
e
)
Toluene.—
IRU *& UHVLGXH DQDO\VLV
(
f
)
Water.—
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(
g
)
Anhydrous sodium sulfate (Na
2
SO
4
).—
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& IRU K DQG WKHQ VWRUHG LQ D GHVLFFDWRU EHIRUH
XVH 1D
2
62
4
prepared and stored as indicated can be used for
1 month from preparation).
(
h
)
Silica gel SPE column.—
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naphthalene in the sample) are still acceptable if the source of
contamination could not be eliminated.
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&
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DQK\GURXV 1D
2
62
4
.
(
i
)
Anhydrous magnesium sulfate (MgSO
4
).—
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& IRU K DQG WKHQ VWRUH LQ D
GHVLFFDWRU EHIRUH XVH 0J62
4
prepared and stored as indicated
can be used for 1 month from preparation).
Note
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can be used.
(
j
)
Sodium chloride (NaCl).—
(
k
)
Helium 5.0 or better, nitrogen 4.0 or better.
(
l
)
Polypropylene centrifuge tubes.—
P/
(
m
)
Glass Pasteur pipet.—
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LQ KRXVH SUHSDUDWLRQ RI VLOLFD JHO PLQLFROXPQV
(
n
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Syringes/pipets.—
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and transfer of appropriate volumes for standard solution
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(
o
)
9ROXPHWULF ÀDVNV ²
± P/
(
p
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Glassware for evaporation steps
.
—
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Table 2014.08B. (
continued
)
PAH
1R RI
laboratories
1R RI
replicates
Mean
concn, μg/kg
Mean
recovery, % s
r
, μg/kg s
R
, μg/kg RSD
r
, % RSD
R
, % HorRat
9
18
Naph
8
16
9
18
8
16
Phe
8
16
9
18
9
18
Pyr
9
18
9
18
8
16