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G
olay
&
M
oulin
:
J
ournal of
AOAC I
nternational
V
ol
.
99, N
o
.
1, 2016
221
Acknowledgments
We would like to specially thank Erik Konings, Christopher
Blake and Francesca Giuffrida at Nestlé Research Centre
(Lausanne, Switzerland), Jaap Evers and Steve Holroyd at
Fonterra (Waitoa, New Zealand), Darryl Sullivan at Covance
Laboratories (Madison WI), Robert Rankin at Infant Nutrition
Council of America (Washington DC), Marc Hill at Abbott
Nutrition (Columbus, OH),Aurélie Dubois-Lozier, at International
Dairy Federation (Brussels, Belgium), Marcel De Vreeze, at the
Netherlands Standardization Institute (Delft, The Netherlands),
Giovanna Contarini at Centro di Ricerca per le Produzioni
Foraggere e Lattiero-Casearie (CREA-FLC) (Lodi, Italy) and
Harrie van den Bijgaart at Qlip (Zutphen, The Netherlands)
for their great support for this project and/or for initiating the
collaboration between AOAC and ISO-IDF organizations.
We thank the 18 participant laboratories and their associates
for participating in the study:
Javier Fontecha and Luís M. Rodríguez y Alcalá, Institute of
Food Science Research CSIC-UAM (Madrid, Spain)
Tiina Ritvanen, Finnish Food Safety Authority EVIRA
(Helsinki, Finland)
Philippe Trossat and Stephane Chappaz, Actalia (Mamirolle,
France)
Daryl Sullivan and Sarah Kanable, Covance Laboratories
(Madison, WI)
Martin Alewijn, RIKILT Institute of Food Safety
(Wageningen, The Netherlands)
Hans Cruijsen and Nicolae Portinga, R., Friesland Campina,
(Leeuwarden, The Netherlands)
Ute Braun and Hans Tober, MUVA (Kempten, Germany)
Iain McGrail, Fonterra (Waitoa, New Zealand)
Cynthia Srigley, U.S. Food and DrugAdministration (College
Park, MD)
Pierluigi Delmonte, U.S. Food and Drug Administration
(College Park, MD)
Shay Phillips, Sherri VanDenBerg and Nicole Keller, Mead
Johnson Nutrition (Zeeland, MI)
Carolin Lehmann, Irina Scholl and Annika Braun, Institut
Kirchhoff (Berlin, Germany)
Table 5. Method performance versus SMPR 2012.011
Parameter
SLV
MLT
a
SMPR 2012.011
b
Matrixes
SLV test Matrixes kit
(12 samples)
SLV test Matrixes kit
(6 samples)
c
All forms of infant, adult, and/or
pediatric formula (powders, ready-to-
feed liquids, and liquid concentrates)
LOQ, g/100 g
0.001
d
0.001
d
≤0.001
d
Analytical range, g/100 g
0.001–7.94
d
≤0.001–8.00
d
Recovery
100.0–102.9% (for C18:2
n
-6 and
C18:3
n
-3)
90–110% for labeled fatty acids;
80–110% of mean spiked recovery
over the range of the assay
Repeatability (RSD
r
), %
≥3.0
d
<1.3
2.5
≤2.0
≥0.5 to <3.0
d
<1.7
1.2–4.8
≤4.0
≥0.05 to <0.5
d
<1.8
1.9–7.0
≤7.0
≥0.005 to <0.05
d
<3.5
2.1–(16.6)
e
<10
≤0.001 to <0.005
d
<6.2
<15
Intralaboratory precision (RSD), %
≥3.0
d
<1.1
Not specified
≥0.5 to <3.0
d
<1.7
≥0.05 to <0.5
d
<2.9
≥0.005 to <0.05
d
<6.3
≤0.001 to <0.005
d
<7.0
Reproducibility (RSD
R
), %
≥3.0
d
Not done
5
≤4
≥0.5 to <3.0
d
1.9–9.0
≤8
≥0.05 to <0.5
d
4.9–8.4
≤15
≥0.005 to <0.05
d
3.6–(42.54)
e
<25
≤0.001 to <0.005
d
<40
a
MLT = Multilaboratory testing.
b
SMPR 2012.011, Final, September 29, 2012. Concentrations apply to (
1
) “ready-to-feed” liquids “as-is”; (
2
) reconstituted powders (25 g into 200 g
water); and (
3
) liquid concentrate diluted 1:1 by weight.
c
The table is valid only for SPIFAN Samples 7 to 12.
d
Grams of fatty acids per 100 g reconstituted final product.
e
Results in parentheses correspond to TFA amounts found in SPIFAN materials in very low amounts.
205