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G
olay
&
M
oulin
:
J
ournal of
AOAC I
nternational
V
ol
.
99, N
o
.
1, 2016
This calculation can be performed only when the fat content
is determined with an appropriate and validated fat extraction
method. Do not use the declared fat value for the expression
of fatty acids on finished products due to possible imprecision
between fat labeled and fat extracted.
(
4
)
Sum of class or group of fatty acids.
—Calculate the mass
fraction of all fatty acids included in a group or in a class of fatty
acids by the simple addition of individual fatty acids results
corresponding to each class or group (expressed in grams fatty
acids (FA) per 100 g product):
=
+
+
∑
(
/ 100
/ 100
/ 100 )
1
2
3
FA gFA g gFA g gFA g
i
i
i
Table 2012.13. Stoichiometric factors for converting FAMEs to fatty acids
Chain length
Position of
unsaturation
(terminal methyl
carbon)
Configuration Group Abbrev.
FAME mol. wt,
g/mol
FA mol. wt, g/mol TAG mol. wt, g/mol
S
i
(FA)
a
C4:0
SFA
102.1
88.1
302.4
0.863
C6:0
SFA
130.2
116.2
386.5
0.892
C8:0
SFA
158.3
144.2
470.7
0.911
C10:0
SFA
186.3
172.3
554.9
0.925
C12:0
SFA
214.4
200.3
639.0
0.935
C14:0
SFA
242.4
228.4
723.2
0.942
C14:1
ω-5 (or
n
-5)
Cis
MUFA
b
240.4
226.4
717.1
0.942
C15:0
SFA
256.4
242.4
765.3
0.945
C15:1
ω-5 (or
n
-5)
Cis
MUFA
254.4
240.4
759.2
0.945
C16:0
SFA
270.5
256.4
807.3
0.948
C16:1
ω-7 (or
n
-7)
Cis
MUFA
268.5
254.4
801.3
0.948
C17:0
SFA
284.5
270.5
849.4
0.951
C17:1
ω-7 (or
n
-7)
Cis
MUFA
282.5
268.4
843.4
0.950
C18:0
SFA
298.5
284.5
891.5
0.953
C18:1 TFA
Trans
c
296.5
282.5
885.5
0.953
C18:1
ω-9 (or
n
-9)
Cis
MUFA
296.5
282.5
885.5
0.953
C18.2 TFA
Trans
c
294.5
280.5
879.4
0.952
C18:2
ω-6 (or
n
-6)
Cis
PUFA
LA
d
294.5
280.5
879.4
0.952
C18:2 CLA
ω-6 (or
n
-6)
Cis
/
trans
PUFA
CLA
294.5
280.5
879.4
0.952
C18:3
ω-6 (or
n
-6)
Cis
PUFA
292.5
278.4
873.4
0.952
C18:3 TFA
Trans
c
292.5
278.4
873.4
0.952
C18:3
ω-3 (or
n
-3)
Cis
PUFA
ALA
e
292.5
278.4
873.4
0.952
C20:0
SFA
326.6
312.5
975.7
0.957
C20:1
ω-9 (or
n
-9)
Cis
MUFA
324.6
310.5
969.6
0.957
C20:2
ω-6 (or
n
-6)
Cis
PUFA
322.5
308.5
963.6
0.957
C20:3
ω-6 (or
n
-6)
Cis
PUFA
320.5
306.5
957.5
0.956
C20:3
ω-3 (or
n
-3)
Cis
PUFA
320.5
306.5
957.5
0.956
C20:4
ω-6 (or
n
-6)
Cis
PUFA
ARA
f
318.5
304.5
951.5
0.956
C20:5
ω-3 (or
n
-3)
Cis
PUFA
EPA
g
316.5
302.5
945.4
0.956
C22:0
SFA
354.6
340.6
1059.9
0.960
C22:1
ω-9 (or
n
-9)
Cis
MUFA
352.6
338.6
1053.8
0.960
C22:2
ω-6 (or
n
-6)
Cis
PUFA
350.6
336.6
1047.8
0.960
C22:6
ω-3 (or
n
-3)
Cis
PUFA DHA
h
342.5
328.5
1023.6
0.959
C24:0
SFA
382.7
368.7
1144.0
0.963
C24:1
ω-9 (or
n
-9)
Cis
MUFA
380.7
366.6
1137.9
0.963
a
S
i
(FA) = Stoichiometric factor to convert FAME
i
to FAi.
b
MUFA = Monounsaturated fatty acid.
c
Does not include TFAs in MUFA and PUFA sums.
d
LA = Linoleic acid.
e
ALA = α-Linolenic acid.
f
ARA = Arachidonic acid.
g
EPA = Ecosapentaenoic acid.
h
DHA = Docosahexaenoic acid.
202