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Biophysical Society 59
th
Annual Meeting, Baltimore, Maryland
Sunday, February 8
7:30
am
–8:30
am
, R
oom
327/328/329
Postdoctoral Breakfast
Supported by the Burroughs Wellcome Fund
This breakfast presents an opportunity for postdoctoral members of the
Society to meet and discuss the issues they face in their current career
stage. This year, there will be an emphasis on challenges and opportunities
in publishing. Members of the Early Careers Committee will be available
to answer questions about how the Committee serves postdocs in the
biophysical community. Limited to the first 100 attendees.
7:30
am
–9:00
am
, H
all
C, R
oom
A
Exhibitor Presentation
FEI Company
FEI Cryo-TEMWorkflow Solutions: A New Era for 3D
Structural Biology
A new frontier exists in unraveling interactive biological and biochemical
processes and pathways at the macromolecular level. Of critical
importance is the three-dimensional visualization of macromolecular
structures and molecular machines in their native functional state. Three
techniques play a major role in orchestrating this.
Nuclear magnetic resonance (NMR) has the capability to study specific
protein domains or fragments and their functional role in protein folding
and dynamics and in ligand binding whereas X-Ray crystallography
(XRD) allows visualizing high-resolution but more static 3D structures
of apo and liganded proteins, mainly in a monomeric or dimeric state
after crystallization. To unravel more physiologically relevant situations
however, it is essential to visualize multimeric complexes in their tertiary
and quaternary state and their interaction with other complexes. By
performing typical cryo-TEM applications like single particle analysis
or tomography, this can be achieved. In this so-called translational
methodology, cryo-TEM thus provides complementary information to
NMR and XRD that can be crucial for drug discovery, e.g. in terms of a
better understanding of the mechanism of action inferred from the EM
structure of the physiologically relevant complex. This will eventually
contribute to answer real biologically as well as medically relevant
questions.
Latest developments in the cryo-TEM workflow have brought the 3 major
structural biology technologies closer together. Now, finally, a continuum
has been reached on all important aspects with regards to resolution
and macromolecular scales which allows for the full deployment of the
combination of these technologies.
Here, we will illustrate the historical context of these technologies with
respect to one another and show how latest developments have reached
the critical requirements to fully unleash the power of structural biology
in not just answering fundamental questions, but actually contribute to
curing diseases and improving health. Also, we will discuss the future of
structural biology based on the latest developments of the FEI workflow
and its components with a special focus on the advances in contrast
enhancement (phase plates) and (direct electron) detection.
Presenter
Chris Arthur, Applications Engineer, FEI Company
7:30
am
–5:00
pm
, C
harles
S
treet
L
obby
Registration/Exhibitor Registration
8:00
am
–6:30
pm
, R
oom
301/302/303
Career Center
8:00
am
–10:00
pm
, H
all
C
Poster Viewing
8:15
am
–10:15
am
, B
allroom
I
Symposium
Regulated Protein Bridges Connecting
Membranes: STIM Proteins in
Cellular Signaling
Chair
Richard Lewis, Stanford University
52-S
ymp
8:15
am
SINGLE-MOLECULE STUDIES OF THE ER CALCIUM SENSOR
STIM1.
Richard Lewis
53-S
ymp
8:45
am
TUNING THE TAPS: STIM1 AND STIM2 REGULATORY
MECHANISMS.
Barbara A. Niemeyer
54-S
ymp
9:15
am
GATING MECHANISMS OF STORE-OPERATED CRAC
CHANNELS.
Murali Prakriya
55-S
ymp
9:45
am
THE MECHANICS OF STIM-ORAI COMMUNICATION.
Patrick Hogan
8:15
am
–10:15
am
, B
allroom
II
Symposium
Mechanosensors
Chair
Marcos Sotomayor, Ohio State University
56-S
ymp
8:15
am
THE MINIMAL CADHERIN-CATENIN COMPLEX BINDS TO
ACTIN FILAMENTS UNDER FORCE. Craig Buckley, Jiongyi Tan,
William Weis, W. James Nelson,
Alexander Dunn
57-S
ymp
8:45
am
MECHANISMS AND MECHANOSENSITIVITY: EXCEPTIONAL
CADHERINS FOR HEARING AND BALANCE.
Marcos Sotomayor
58-S
ymp
9:15
am
MECHANICAL FORCES IN B CELL ACTIVATION.
Pavel Tolar
59-S
ymp
9:45
am
NAVIGATING A MAZE - SENSING AND RESPONDING TO
MECHANICAL OBSTACLES DURING CELLULAR INVASIVE
GROWTH. Carlos Agudelo, Amir Sanati Nezhad, Mahmood Ghanbari,
Muthukumaran Packirisamy,
Anja Geitmann
8:15
am
–10:15
am
, B
allroom
III
Platform
Molecular Simulation:
Structure and Interactions
Co-Chairs
Oliver Beckstein, Arizona State University
Alexander Shug, Karlsruhe Institute of Technology, Germany