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© 2015 AOAC INTERNATIONAL
AOAC SMPR 2015.004
Standard Method Performance Requirements
SM
(SMPRs) for Total Vitamin B
3
(Niacin) in Infant and
Adult/ Pediatric Nutritional Formula
Intended Use: Reference Method for Dispute Resolution
1 Applicability
Determinations of total vitamin B
3
(niacin) in all forms of infant,
adult, and/or pediatric formula (powders, ready-to-feed liquids, and
liquid concentrates). Total B
3
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(CAS No. 59-67-6) and nicotinamide (CAS No. 99586-65-9), and
reported as niacin.
2 Analytical Technique
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performance requirements is acceptable.
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number of replicate measured quantity values and a reference
quantity value.
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quantitative result.
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amide, CAS No. 99586-65-9.
4 Method Performance Requirements
See
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5 System Suitability Tests and/or Analytical Quality Control
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6 Reference Material(s)
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infant/adult nutritional powder prepared by a manufacturer of
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7 Validation Guidance
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8 Maximum Time-to-Result
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Approved by AOAC Stakeholder Panel on Infant Formula and
Adult Nutritionals (SPIFAN). Final Version Date: April 17, 2015.
Effective Date: April 17, 2015.
Table 1. Method performance requirements
a
Analytical range
200–10000
b
Limit of quantitation (LOQ)
b
Recovery
90–110%
Repeatability (RSD
r
)
Reproducibility (RSD
R
)
a
Concentrations apply to: (a) “ready-to-feed” liquids “as is”; (b)
reconstituted powders (25 g into 200 g water); and (c) liquid concentrates
diluted 1:1 by weight.
b
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