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© 2015 AOAC INTERNATIONAL
AOAC SMPR 2015.005
Standard Method Performance Requirements
SM
(SMPRs) for Total Vitamin B
6
(Pyridoxine) in Infant
and Adult/ Pediatric Nutritional Formula
Intended Use: Reference Method for Dispute Resolution
1 Applicability
Determinations of
total vitamin B
6
(pyridoxine) in all forms
of infant, adult, and/or pediatric formula (powders, ready-to-feed
liquids, and liquid concentrates). Total B
6
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pyridoxine(CAS No. 65-23-6), pyridoxal (CAS No. 66-72-8),
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corrected and expressed as pyridoxine.
2 Analytical Technique
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quantity value.
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quantitative result.
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pyridine in rats.
See
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pyridoxine: 65-23-6).
4 Method Performance Requirements
See
7DEOH
5 System Suitability Tests and/or Analytical Quality Control
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6 Reference Material(s)
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vitamin B
6
(pyridoxine).
7 Validation Guidance
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SM
.
8 Maximum Time-to-Result
No maximum time.
Approved by AOAC Stakeholder Panel on Infant Formula and
Adult Nutritionals (SPIFAN). Final Version Date: April 17, 2015.
Effective Date: April 17, 2015.
Table 1. Method performance requirements
a
Analytical range
10–2000
b
Limit of quantitation (LOQ)
b
Recovery
90–110%
Repeatability (RSD
r
)
Reproducibility (RSD
R
)
a
Concentrations apply to: (a) ‘ready-to-feed” liquids “as is”; (b)
reconstituted powders (25 g into 200 g water); and (c) liquid concentrates
diluted 1:1 by weight.
b
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