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Determination of B-Vitamins in Infant Formula and Adult/Pediatric Formulas

ESI LC-MSMS

4

Table-4

QQQ Parameters:

Compund

Name

Precursor

Ion

Product ion

Dwell

Time

Fragmentor

Voltage

CE

Cell Acc.

Voltage

Thiamine

265.2

122.2, 81.3

40

92

10, 30

7

Riboflavin

377.1

243.2, 198.1,

172.1

40

100

20, 35, 40

7

Niacinamide

123.2

80.3, 53.3

40

85

20, 30

7

Nicotinic acid

124.2

80.1, 78.1, 53.1

40

50

20, 20, 32

7

Pantothenic

acid

220.1

202.1, 90.0,

71.9

40

100

10, 10, 15

7

Pyridoxine

170.2

152.1, 134.1,

77.3

40

55

10, 20, 35

7

Biotin

245.2

227.3, 105.2,

97.1

45

110

10, 30, 30

7

Folic acid

442.2

295, 176, 120

45

110

10, 40, 40

7

d) Calculations:

(A

s

- A

b

) * V

s

* DF

Result (mg/100g) =

W

s

* 10000

A

s

= Sample concentration in μg/L, from calibration curve

A

b

= Blank concentration in μg/L, from calibration curve

V

s

= Sample Makeup Volume

W

s

= Sample weight in grams

DF

S

= Sample dilution factor (Other than V

s and

W

s

)

G.

References:

a)

AOAC official methods of analysis (AOAC 2004.07 , AOAC 2012.16 )

b)

BS EN 14122:2014, Food Stuffs

Determination of Vitamin B1 by HPLC

c)

BS EN 14164:2014, Food Stuffs

Determination of Vitamin B6 by HPLC

d)

BS EN 15652:2009, Food Stuffs

Determination of Niacin by HPLC

e)

Agilent application note for “Determination of multi water soluble vitamins by LC

-

MS/MS”.

BVit-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE